Talk:EcoRI

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Untitled[edit]

We may just want to redirect this to restriction enzyme... – Clockwork Soul 03:11, 9 Apr 2005 (UTC)

EcoR1 (sometimes spelled with a roman one as in EcoRI) -- Make that always spelled. I've never seen it done differently, except from students who've only heard it said and not seen it written. 128.104.112.77 23:07, 10 May 2005 (UTC)[reply]

I have edited the article to replace the reference to the original enzyme-DNA cocrystal structure published in Science in 1986. The structure published in that paper was later found to be incorrect by the group that published it, which explains why biochemical data gathered by researchers in other laboratories were not consistent with the published structure. A revised structure that has consistently been supported by other biochemical research was published as a Technical Comment in Science in 1990, and this latter publication is the appropriate journal reference for the structure. —Preceding unsigned comment added by 198.160.190.11 (talk) 22:33, 22 August 2008 (UTC)[reply]

reference strangeness[edit]

If someone can explain to me what's going on with the references, then I can fix them if no-one else knows how... --Arkelweis (talk) 01:20, 24 September 2009 (UTC)[reply]

Proposals for improvements?[edit]

I'm supposed to edit a scientific article for my molecular bio class, but I wanted to start out with a small article. Please let me know what you think! In the introduction, it says that EcoRI "creates 4 nucleotide sticky ends". This may be misleading in a couple ways. From that phrase, I couldn't tell whether the sticky ends were created on one of the strands in four different locations, or if it meant the length of the sticky ends was 4 base pairs. It should be made clear that EcoRI makes two cuts, each cut creating a sticky end. It should go on to describe what a sticky end actually is. The part "5' end overhangs of AATT" was a little vague, as well. Maybe here, it could be specified that the cut was made between the G and the A, as shown in the figure. In the figure of the EcoRI recognition site, it might be ideal to include the 5' and 3' ends for directionality. Maybe also explain that restriction enzymes tend to be palindromic (a term that I learned with this class, although it might have another term) with their complementary sequences. It may be useful to include an example of EcoRI being used for genetic deletion from, say, a target plasmid. Bwongcha (talk) 11:20, 13 February 2016 (UTC)[reply]

Wiki Education- Course Assignment[edit]

Hello Wikipedia community, This is Fadi Asrawi, a Molecular Biology master student and, this is the article that assigned to me under the Recent Developments in Biotechnology Course. Further details are available on the course page. Fadi Asrawi (talk) 21:03, 21 January 2023 (UTC)[reply]